Enzyme immunoassay (ELISA) for the determination of soluble human leptin receptor (sLEP-R) in human serum or plasma. For research use only, not for use in diagnostic procedures.<br><br>
The adipokine leptin realizes signal transduction via four different leptin receptor (LEP-R) isoforms. The amount of functionally active LEP-R, however, is affected by constitutive shedding of the extracellular domain. The product of the cleavage process, the so-called soluble leptin receptor (sLEP-R, soluble Leptin receptor), is the main binding protein for leptin in human blood and modulates its bioavailability.
- Assay Description:
- 2 hour incubation (RT) + 2 hr. (RT) + 30 min. (RT) + 30 min. (RT) = 5 hour total incubation time
- Catalog number:
- R07
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- 2 controls, lyophilized
- design:
- Enzyme immunoassay (ELISA) technique
- FDA Status:
- For research use only, not for use in diagnostic procedures
- MSDS:
- Available upon request
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Protocol:
- Sample types:
- Serum or plasma
- Sample volume:
- 100 μL of properly diluted unknowns (1:5 dilution) / determination
- standards:
- 8 standards, lyophilized
- Standard range:
- 0 / 3.125 - 150 ng/mL
- storage:
- 2 - 8 °C
- sensitivity:
- 0.0385 ng/mL
- Species:
- Human
