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Leptin ELISA

$1,946.00
SKU:
L07
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Ligand-Enzyme Immunoassay (ELISA) for the determination of functional, receptor binding Leptin in human serum and plasma (EDTA, Heparin). For research use only, not for use in diagnostic procedures.<br><br>

Leptin a hormone of 146 amino acids shows a molecular weight of 16 kDa (P41159). It consists of four anti-parallel α-helices. Leptin is primarily produced by adipocytes and thus provides a signal to the energy state of the organism. By binding to the leptin receptor (P48357) it influences the activity of the JAK -STAT pathway and thereby regulates energy metabolism, especially the food intake. Leptin levels show a circadian variation (+/- 30%) and are dependent on BMI, pubertal status, and gender. In addition to the expression itself, the bioavailability of leptin, is regulated via a binding protein, as with many hormones with type I cytokine receptors. The binding protein is the extracellular domain of the Leptin receptor formed by proteolytic cleavage of the receptor be metalloproteases (ADAM 10/17).<br><br>

In healthy persons food intake is reduced by increasing concentrations of leptin and by falling SOB-R quantities. In various pathological situations, this regulatory circuit is however interrupted: e.g. the leptin levels in the circulation of obese persons are increased, but resulting in no satiety. This phenomenon, known as leptin resistance, could have its cause in a reduction in the number of leptin receptors or in influencing the intracellular signal transduction by other parameters.<br><br>

Lately it was shown that a naturally occurring transversion in the leptin gen (c.298G → T) results in an amino acid exchange of asparagine to tyrosin in position 100 (pD100Y). An in vitro cell culture model revealed that this mutated leptin was still secreted but unable to bind to the receptor and therewith unable to exert intracellular signaling. In vivo this mutation results in disturbance of food intake regulation and in consequence in extreme obesity. Under treatment with recombinant human leptin food intake behavior normalized and concomitantly body weight was reduced significantly.

Assay Description:
1 hour incubation (RT) + 1 hour (RT) + 30 min. (RT) + 30 min. (RT) = 3 hours total incubation time
Catalog number:
L07
configuration:
96 Determinations, 12x8 removable strips
controls:
2 controls, lyophilized
design:
Ligand-Enzyme Immunoassay (ELISA) technique
FDA Status:
For research use only, not for use in diagnostic procedures
MSDS:

MSDS - ELISA
MSDS - Controls

notes:
The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
Protocol:

Protocol

Sample types:
Human serum and plasma (EDTA, Heparin)
Sample volume:
100 µL of properly diluted (1:20) unknown / determination
standards:
8 standards, lyophilized
Standard range:
0 / 0.05 - 6.00 ng/mL
storage:
2 - 8°C
sensitivity:
< 0.01 ng/mL
Species:
Human