Solid phase sandwich ELISA designed for the determination of T-Cadherin (30k, 130k) in human serum and EDTA-plasma. For research use only, not for use in diagnostic procedures. <br><br>
T-Cadherin is a protein that binds to Adiponectin (APN), an adipocyte-secreted factor.
APN binds to and accumulates in aorta, heart, skeletal muscle, and mesenchymal stem cells in systemic tissues via T-Cadherin, and is thought to promote exosome production and exert a protective effect on organ tissues. In addition, SNPs near the T-Cadherin gene are strongly associated with glucose intolerance and cardiovascular disease risk in humans.<br><br>
Recently, it was reported that soluble T-Cadherin exists in human blood in three forms: 130kDa, 100kDa, and 30kDa. These three soluble forms of T-Cadherin correlate with various clinical parameters in patients with type 2 diabetes, and their concentrations change rapidly in the acute phase of myocardial infarction, so they are expected to be useful as biomarkers.
In addition, soluble T-Cadherin has been found to promote the proliferation of pancreatic beta cells and inhibit the decline in insulin secretory capacity, and its function as a humoral factor is also in focus.<br><br>
We provide 3 ELISA kits that measure (1) 130kDa molecular species only, (2) 30kDa + 130kDa molecular species, and (3) 100kDa + 130kDa molecular species.
- Assay Description:
- 90 min. (37°C) + 30 min. (2-8°C) + 30 min. (RT) = 2 hours total incubation time
- Catalog number:
- 27365
- Configuration:
- 96 Determinations, 12x8 removable strips
- Controls:
- None provided
- Design:
- Solid phase sandwich ELISA
- FDA Status:
- For research use only, not for use in diagnostic procedures
- MSDS:
- Please inquire
- Protocol:
- Notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Sample types:
- Human Serum, EDTA-plasma
- Sample volume:
- 100 uL of properly diluted unknown / determination
- Standards:
- 7 standards, serially diluted from 1 prepared lyophilized standard
- Standard Range:
- 0/ 0.02 - 1.5 ng/mL
- Storage:
- 2 - 8 °C
- Sensitivity:
- 0.005 ng/mL
- Species:
- Human
- Pamphlets:
