Assay Description
1 hour incubation (37°C) + 30 min. (RT) + 30 min. (RT) = 2 hours total incubation time
Catalog number
COMPL 300 RUO
configuration
One frame with break apart wells (4x8x3). 4 blue coloured strips for classical pathway (CP), coated with human IgM. 4 green coloured strips for MBL pathway (MP), coated with mannan. 4 red coloured strips for alternative pathway (AP), coated with LPS.
controls
1 negative control (ready to use) and 1 positive control (lyophilized) included
design
Enzyme immunoassay (ELISA) technique
FDA Status
For research use only, not for use in diagnostic procedures
MSDS
Please inquire
notes
The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
Sample types
Human serum
Sample volume
100 µL of properly diluted serum / determination (please see protocol, section labelled "Dilution of Serum")
standards
The negative and positive controls can be used in such a way to calculate complement activity
Standard range
The negative and positive controls can be used in such a way to calculate complement activity
storage
The reagents should be stored at 2 - 8° C except the positive control. The positive control should be stored at -20° C
Species
Human