Solid phase sandwich ELISA for the determination of Gd-IgA1 (Galactose-deficient IgA1) in human serum, EDTA-plasma, and urine. For research use only, not for use in diagnostic procedures.
Galactose-deficient IgA1 (Gd-IgA1) attracts a lot of attentions as a critical effector molecule in the pathogenesis and progression of IgA nephropathy (IgAN) in recent studies. It has been suggested that several O-link glycans modified regions exist in the heavy chain hinge region of human IgA1 molecule andGd-IgA1 circulates in blood stream of test samples with the pathological condition of IgAN. The measuring system using snail (helix aspersa; HAA) lectin that is extracted from snail has been used in past numerous studies and it was revealed that serum levels of Gd-IgA1 in samples with IgAN is significantly elevated compared with the level of healthy subjects with renal diseases other than IgAN. Thus, the importance and purpose of measuring serum Gd-IgA1 level have been gradually recognized from such studies.
However, since the measuring system used HAA lectin is not suitable for measuring multiple and massive samples in large scale studies due to its instability of glycan-recognizing activity, development of alternative measuring system that can quantitatively measure human Gd-IgA1 in serum with stable and reliable data has been considered as an essential and urgent matter. This IBL ELISA kit using the monoclonal antibody that specifically recognizes galactose-deficient hinge sequence of human Gd-IgA1 is a lectin non-dependent measuring system that can quantitatively measure Gd-IgA1 in human serum, which is also suitable for large scale studies because of its stability.
- Assay Description:
- 1 hour incubation (RT) + 30 min. (RT) + 30 min. (RT) = 2 hours total incubation time
- Catalog number:
- 27600
- configuration:
- 96 Determinations, 12x8 removable strips
- Protocol:
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- design:
- Solid phase sandwich enzyme-linked immunosorbent assay (ELISA)
- MSDS:
- FDA Status:
- For research use only, not for use in diagnostic procedures
- Other names:
- Galactose-deficient IgA1
- Sample types:
- Human serum , EDTA-plasma, and Urine
- Sample volume:
- 50 µL of properly diluted unknown / determination
- standards:
- 7 standards, serially diluted from 1 prepared lyophilized standard
- Standard range:
- 1.56 - 100.0 ng/mL
- storage:
- 2 - 8 °C
- sensitivity:
- 0.488 ng/mL
- Species:
- Human
- Additional info:
- FAQ:
- References:
