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Angiotensinogen Detection | ELISAs & Antibodies

Jun 5th 2019

Angiotensinogen Detection

Angiotensinogen (also known as serpin peptidase inhibitor, clade A, member 8; Serpin A8, Ang, Ang II, PAT, and ANRT) is a 452-amino acid hormone precursor produced by the liver and is implicated in blood pressure control.

Urinary excretion of angiotensinogen in a rat hypertension model indicates that this protein is involved in the intrarenal renin-angiotensin system (RAS).

In response to lowered blood pressure, the enzyme renin cleaves angiotensinogen to produce angiotensin-I, which is further cleaved by angiotensin-converting enzyme (ACE) to yield the physiologically active peptide angiotensin-II. 

Angiotensin-II stimulates aldosterone release, promoting sodium retention in the kidney and an increase in blood pressure. Angiotensin II is also implicated in the regulation of insulin-like growth factor 1 (IGF-1) expression.

ELISAs for Angiotensinogen Detection

These ELISA kits are solid-phase sandwich ELISAs that take advantage of two highly specific antibodies; one antibody is coated onto the ELISA plate and the other is HRP-conjugated.

The Human Total and Intact Angiotensinogen Assay Kits are ELISA kits that can be used with serum, EDTA-plasma, urine, or cell culture media.

Antibodies for Angiotensinogen Detection

These affinity-purified antibodies recognize either mouse/rat or human angiotensinogen protein. The antibodies can be used for Western blot (WB) detection or immunohistochemical (IHC) detection of angiotensinogen protein.

Antigen
Cat. #
Application
Species

(Clone) and Source

Cross‑Reactivity

Angiotensinogen 27412 ELISA Human

Capture:
(72)
Mouse IgG

Detect: HRP
(601)
Mouse IgG MoAb

Shows ≤0.1 cross‑reactivity against angiotensin proteins I–IV, angiotensin (1–7), angiotensin (1–9), human albumin, human IgG, and human angiopoietin-like 3
Intact Angiotensinogen
27742
ELISA Human Capture: 
AGT(A1) Rabbit IgG

Detect:

AGT(601) Mouse IgG

Specifically detects Human Intact Angiotensinogen.

Angiotensinogen (C-terminus; 394–485) 10417 WB Human (104AT 601.2.80)
Mouse IgG MoAb
n/a
Mouse angiotensinogen (C-terminus) 28101 IHC WB Mouse,  Rat
(405)
Rabbit IgG
n/a

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