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Mayo Clinic Study Uses IBL-America's sAPP Alpha ELISA

October 11, 2017

Endothelium-specific amyloid precursor protein deficiency causes endothelial dysfunction in cerebral arteries

LV d'Uscio, T He, AV Santhanam, ZS Katusic - Journal of Cerebral Blood Flow & …, 2017

The exact physiological function of amyloid-β precursor protein (APP) in endothelial cells is unknown. Endothelium-specific APP-deficient (eAPP−/−) mice were created to gain new insights into the role of APP in the control of vascular endothelial function. Endothelium-dependent relaxations to acetylcholine were significantly impaired in basilar arteries of global APP knockout (APP−/−) and eAPP−/− mice (P < 0.05). In contrast, endothelium-independent relaxations to nitric oxide (NO)-donor diethylamine-NONOate were unchanged. Western blot analysis revealed that protein expression of endothelial nitric oxide synthase (eNOS) was significantly downregulated in large cerebral arteries of APP−/− mice and eAPP−/− mice as compared to respective wild-type littermates (P < 0.05). Furthermore, basal levels of cyclic guanosine monophosphate (cGMP) were also significantly reduced in large cerebral arteries of APP-deficient mice (P < 0.05). In contrast, protein expression of prostacyclin synthase as well as levels of cyclic adenosine monophosphate (cAMP) was not affected by genetic inactivation of APP in endothelial cells. By using siRNA to knockdown APP in cultured human brain microvascular endothelial cells we also found a significant downregulation of eNOS mRNA and protein expressions in APP-deficient endothelium (P < 0.05). These findings indicate that under physiological conditions, expression of APP in cerebral vascular endothelium plays an important protective function by maintaining constitutive expression of eNOS.

The #27734 Human sAPPa (highly sensitive) ELISA was used in this study.


Visit Us at the 2017 Society for Neuroscience Annual Meeting

October 9, 2017

Society for Neuroscience's 47th annual meeting, Neuroscience 2017, is the world’s largest neuroscience conference for scientists and physicians devoted to understanding the brain and nervous system.

Neuroscience 2017 is where neuroscientists collaborate and network with peers, learn from experts, explore the newest neuroscience tools and technologies, and discover great career opportunities.

The meeting will be held November 11-15 at the Walter E. Washington Convention Center. Join more than 30,000 colleagues from more than 80 countries at the world’s largest marketplace of ideas and tools for global neuroscience. We will be showcasing our Amyloid Beta ( Aβ )and APP ELISA kits and antibodies for Alzheimer’s disease research and many other related products.

Visit us at booth 728 or contact us to set up a meeting!

Visit Us at the UCLA Bioresearch Faire

September 28, 2017

Visit Us At This Show And Discover How IBL-America Can Help You Achieve Your Research Goals

40th Semiannual

Thursday, October 26, 2017

10am - 2:30pm

Ackerman Union, Grand Ballroom, 2nd Floor

University of California, Los Angeles

Contact us to set up a meeting or stop by our booth!

University Study Uses IBL-America's Dengue Virus IgG ELISA

September 20, 2017

Prior Exposure to Zika Virus Significantly Enhances Peak Dengue-2 Viremia in Rhesus Macaques

First in vivo evidence that prior exposure to Zika virus infection can enhance Dengue infection, which has implications for understanding pathogenesis and the development of vaccines.

Serological assays for Dengue Virus specific IgG were performed with IBL-America's kit using serum that was collected longitudinally.


Structural and functional homologies between the Zika and Dengue viruses’ envelope proteins raise the possibility that cross-reactive antibodies induced following Zika virus infection might enhance subsequent Dengue infection. Using the rhesus macaque model we show that prior infection with Zika virus leads to a significant enhancement of Dengue-2 viremia that is accompanied by neutropenia, lympocytosis, hyperglycemia, and higher reticulocyte counts, along with the activation of pro-inflammatory monocyte subsets and release of inflammatory mediators. Zika virus infection induced detectable Dengue cross-reactive serum IgG responses that significantly amplified after Dengue-2 virus infection. Serum from Zika virus immune animals collected prior to Dengue-2 infection showed significant capacity for in vitroantibody dependent enhancement of Dengue-1, 2, 3 and 4 serotypes suggesting that pre-existing immunity to Zika virus could potentially enhance infection by heterologous Dengue serotypes. Our results provide first in vivo evidence that prior exposure to Zika virus infection can enhance Dengue infection, which has implications for understanding pathogenesis and the development of vaccines.


More information about the Dengue Virus IgG ELISA

Visit Us at the 2017 ASBMR Annual Meeting

August 29, 2017

IBL-America will join DIAsource ImmunoAssays® SA at the 2017 ASBMR (Denver, Colorado, September 8-11) and we invite you to visit booth #237.

Discover the Next Generation "Free 25OH Vitamin D Assay". More and more research studies support the importance of free 25OH Vitamin D, suggesting that this fraction of 25OH Vitamin D is a better measurement of Vitamin D status rather than the total 25OH Vitamin D in pregnancy, renal disease, liver disease and intensive care units.

At ASBMR, 5 different posters will illustrate the benefits of measuring free 25OH Vitamin D:

  • Poster MO0473 : "Measurement of (Free) 25OH Vitamin D in Saliva""
  • Poster SU0358 : "Will Free 25OH Vitamin D Measurement Replace The Current Total 25OH Vitamin D Test In IVD Laboratories?"
  • Poster MO0148 : "Free 25 (OH) vitamin D, but not total 25OH Vitamin D, is strongly correlated with gestational age and calcium in normal human pregnancy"
  • Poster SU0303 : "(Free) 25OH Vitamin D measurement and estrogen replacement "
  • Poster MO0418 : "(Free) 25OH Vitamin D in a pediatric population"

We are looking forward to see you in Denver! Contact us to set up a meeting.

More information: http://www.asbmr.org