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Highly Sensitive PGE2 ELISA Kit for Inflammation and Eicosanoid Research

December 11, 2017

Prostaglandin E2 (PGE2)Scientific Background:

Prostaglandin E2 (PGE2) is an extensively studied prostaglandin owing to its predominance in inflammation, cancer, atherosclerosis, autoimmune disease, and sepsis. Oxidation of arachidonic acid by prostaglandin synthases (COX-1 and COX-2) produces prostaglandin H2 (PGH2), which is further metabolized by PGE synthases into its major product, PGE2. PGE2 mediates autocrine and paracrine signaling by binding to G-protein coupled receptors (EP1, EP2, EP3, EP4) on the cell surface, functioning to modulate phospholipase C and adenylate cyclase activity. PGE2 has been of great interest as a therapeutic target, either by modulation of its synthesis by COX inhibitors (NSAIDS) or by modulation of its receptors by downregulation or binding antagonists. PGE2 production in a variety of tissues has been shown to modulate numerous physiological processes including natriuresis in the kidney, smooth muscle elasticity in the vasculature, and the inflammatory response to damaged tissues by monocytes and macrophages.

  • Highly sensitive measurement, detecting as little as 13.4 pg/ml PGE2
  • Higher throughput format with results in <3 hrs for up to 37 samples in duplicate
  • Ready-to-use liquid color-coded reagents reduce errors
  • Reproducible results day-after-day and lot-after-lot

The PGE2 EIA kit is a colorimetric competitive enzyme immunoassay kit with results in < 3 hours. Absorbance is read at 405 nm. Screen inhibitors of COX II activity by measuring the levels of downstream PGE2. Commercially available since 1992, this kit is widely cited in peer-reviewed publications. The non-radioactive ready-to-use liquid color-coded reagents reduce errors.


Product Specification:

Catalog NumberIB09648
SpeciesHuman
DesignCompetitive enzyme immunoassay (ELISA) technique.
Standards8 standards, serially diluted from 1 concentrated standard.
ControlsNone provided.
Sample TypesThis kit is compatible with PGE2 samples in a wide range of matrices after dilution in Assay Buffer - please see 'Sample Handling' section of protocol.
Sample Volume100 μL of properly prepared unknown / determination.
Assay Desc.2 hour incubation (RT) + 45 min. (RT) = 2 hours, 45 min. total incubation time.
Standard Range0 / 39.1 - 2500 pg/mL
Sensitivity13.4 pg/mL
StorageAll components of this kit, except the conjugate and standard, are stable at 4°C until the kit's expiration date. The conjugate and standard must be stored at -20°C.
Configuration96 Determinations, 12x8 removable strips
FDA StatusFor research use only, not for use in diagnostic procedures.


More information: Prostaglandin E2 ELISA

New Citation Uses Our Titin N-Fragment ELISA

December 8, 2017


Diagnostic and clinical significance of the titin fragment in urine of Duchenne muscular dystrophy patients.

Duchenne muscular dystrophy (DMD) is a fatal progressive muscle wasting disease of childhood. Titin in sarcomere is digested by calcium dependent protease. To explore muscle damage in DMD, the urinary concentrations of the N-terminal fragment of titin were determined using a newly developed enzyme linked immune sorbent assay kit. The urinary titin concentrations were normalized to creatinine (Cr). A total of 145 urine samples were obtained at a single Japanese hospital from 113 DMD patients aged 3-29years. Normalized urinary titin concentration was 965.8±1011.9 (Mean±SD) pmol/mg Cr in patients with DMD. This was nearly 700-fold higher than healthy children (1.4±0.8pmol/mg Cr). The concentration was significantly higher in DMD than in BMD patients who had significantly higher urinary titin than normal. Urinary titin in DMD patients tended to decrease with age. The median concentration of urinary titin in the youngest (aged 3-7 years) and oldest (aged ≥16years) groups was 1468.3 and 411.3pmol/mg Cr, respectively, with significant difference. Urinary concentration of titin correlated significantly with serum creatine kinase concentration, the best-known biomarker of DMD. The N-terminal fragment of titin in urine has potential as a diagnostic and clinical biomarker for DMD. Read more.

More information about this assay: Titin Protein N-Fragment ELISA (Cat #27900)

Mayo Clinic Study Uses IBL-America's Amyloid Beta ELISA

November 30, 2017

Distinct spatiotemporal accumulation of N-truncated and full-length amyloid-β42 in Alzheimer’s disease

Accumulation of amyloid-β peptides is a dominant feature in the pathogenesis of Alzheimer’s disease; however, it is not clear how individual amyloid-β species accumulate and affect other neuropathological and clinical features in the disease. Thus, we compared the accumulation of N-terminally truncated amyloid-β and full-length amyloid-β, depending on disease stage as well as brain area, and determined how these amyloid-β species respectively correlate with clinicopathological features of Alzheimer’s disease. To this end, the amounts of amyloid-β species and other proteins related to amyloid-β metabolism or Alzheimer’s disease were quantified by enzyme-linked immunosorbent assays (ELISA)...Read more


Learn more about our Amyloid Beta 1-40 and 1-42 (FL) assays:

Amyloid Beta ( 1-40 ) ( FL ) Aβ

Amyloid Beta ( 1-42 ) ( FL ) Aβ

Product Spotlight | α-Synuclein ELISA

November 28, 2017

α-Synuclein is a 14.5kDa, soluble form protein which consists of 140 amino-acid residue and it is known as an intrinsically disordered protein. It is mainly expressed in cerebral neocortex, hippocampus, nigra, thalamus and metepencephalon. The majority of it exists in the presynaptic terminal and nucleus of neuron cells. It is involved with the signal regulatory system of the synaptic vesicle. α-Synuclein is a main structural component of Lewy bodies, abnormal aggregates of protein that develop inside nerve cells in neurodegenerative diseases such as Parkinson's disease, dementia, multiple system atrophy and other disorders. It is considered that excessive accumulation of α-Synuclein is a main cause of synucleinopathy due to inducing the shedding of dopamine-producing cells.

The concentration of α-Synuclein in human blood or human CSF can be measured by our ELISA kit. This kit is a solid phase sandwich ELISA using 2 kinds of highly specific antibodies. Tetra Methyl Benzidine (TMB) is used as a coloring agent (Chromogen). The strength of coloring is proportional to the quantities of Human α-Synuclein.

For research use only, not for use in diagnostic procedures.

Catalog Number27740
SpeciesHuman
DesignSolid phase sandwich ELISA using 2 kinds of highly specific antibodies.
Standards8 standards, serially diluted from 1 prepared lyophilized standard.
ControlsNone provided.
Sample TypesSerum, EDTA plasma, CSF, and cell culture supernatant.
Sample Volume100 μL of properly diluted unknown / determination.
Assay Desc.Overnight incubation (2-8°C) + 1 hour (2-8°C) + 30 min. (RT) = Overnight + 1 hour, 30 min. total incubation time.
Standard Range0 / 0.16 - 10.00 ng/mL
Sensitivity0.03 ng/mL

More information: α-Synuclein ELISA

Happy Thanksgiving from IBL-America!

November 22, 2017

Wishing you blessings of health, happiness & success this Thanksgiving, and always.

Happy Thanksgiving!!