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APP βCTF

$634.00
SKU:
27776
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Solid phase sandwich ELISA for the determination of Human APP βCTF in cell lysate. For research use only, not for use in diagnostic procedures.<br><br>

Alzheimer’s disease (AD) was first reported by A. Alzheimer, a German neuropathologist in 1907 and is considered as a major factor of senile dementia. It is known that Amyloid β (Aβ; which is a major constituent of senile plaque) is cleaved from Amyloid Precursor Protein (APP; which exists in three main isoforms, APP695, APP751, and APP770) by β-secretase and subsequent γ-secretase. On the one hand, it has been also reported there is βCTF (β-secretase C-terminal fragment), fragments which are cleaved by β-secretase but not by γ-secretase. It is thought that measuring βCTF, in addition to Aβ, soluble APPα and APPβ, also has significance in research of the metabolic pathway of APP. This ELISA can measure human APP βCTF in samples such as cultured cell lysate.

Assay Description:
Overnight incubation (4°C) + 1 hour (4°C) + 30 min. (RT) = Overnight + 1 hour, 30 min. total incubation time
Catalog number:
27776
configuration:
96 Determinations, 12x8 removable strips
controls:
None provided
design:
Solid phase sandwich ELISA using 2 kinds of high specific antibodies
FDA Status:
For research use only, not for use in diagnostic procedures
MSDS:

MSDS

notes:
The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
Protocol:

Protocol

Sample types:
Cell lysate
Sample volume:
100 μL of properly diluted unknown / determination
standards:
8 standards, serially diluted from 1 prepared lyophilized standard
Standard range:
0 / 0.19 - 12 pmol/L
storage:
2 - 8 °C
sensitivity:
0.02 pmol/L
Species:
Human
Products related:
The control set is available for sale on request.
Additional info:

AD Assays and Antibodies

Comprehensive Lineup

References:

References