(Pro)renin receptor/(P)RR is a common receptor protein between renin and prorenin. It has an ability at an equivalent level as renin to bind angiotensinogen and a catalytic activity for conversion from angiotensinogen to angiotensin I once it binds to the (P)RR. Additionally, its intracellular signal transmission promotes once the (P)RR is stimulated by binding with prorenin. Solid phase sandwich ELISA for the determination of soluble (Pro)renin receptor in human serum, EDTA-plasma, and urine. This kit can also be used for the determination of soluble (Pro)renin receptor in mouse and rat serum, EDTA-plasma, and cell culture supernatant. For research use only, not for use in diagnostic procedures.
- Assay Description:
- Overnight incubation (4°C) + 1 hour (4°C) + 30 min. (RT) = Overnight + 1 hour, 30 min. total incubation time
- Catalog number:
- 27782
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- None provided
- design:
- Solid phase sandwich ELISA using 2 kinds of highly specific antibodies
- FDA Status:
- For research use only, not for use in diagnostic procedures
- MSDS:
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Other names:
- (Pro)renin receptor/(P)RR
- Protocol:
- Sample types:
- Human: serum, EDTA-plasma, urine
Mouse and Rat: serum, EDTA-plasma
Cell culture supernatant - Sample volume:
- 100 μL of properly diluted unknown / determination
- standards:
- 8 standards, serially diluted from 1 prepared lyophilized standard
- Standard range:
- 0 / 125 - 8000 pg/mL
- storage:
- 2 - 8 °C
- sensitivity:
- 24 pg/mL
- Species:
- Human, Mouse, Rat
- Additional info:
- References:
- Related Article 1:
- Related Article 2:
