Sandwich enzyme immunoassay (ELISA) for the determination of prolactin (PRL) in human serum. FDA exempt, can be used for in-vitro diagnostics.
Prolactin (PRL) is a polypeptide hormone synthesized by the lactotropic cells of the anterior pituitary gland. Structurally, it is similar to two other polypeptide hormones namely, growth hormone and placental lactogen. PRL is a polypeptide containing 199 amino acids, while growth hormone and placental lactogen each have 191 amino acids. There is approximately 100 µg of prolactin in the human pituitary gland, which is a very small amount when compared to growth hormone, which is present at 8–10 mg.
The target organ of prolactin is the breast (mammary gland). Its main physiological action is not only to initiate but also to sustain lactation. The hypothalamus secretes dopamine, which has a direct effect of inhibition of the secretion of PRL. If dopamine is not available or absent the secretion of PRL is autonomous.
- Assay Description:
- 1 hour incubation (RT) + 15 min. (RT) = 1 hour, 15 min. total incubation time
- Catalog number:
- IB59110
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- 2 controls, ready to use
- design:
- Sandwich enzyme immunoassay (ELISA) technique
- FDA Status:
- 510(k) exempt - IVD
- MSDS:
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Protocol:
- Sample types:
- Human serum
- Sample volume:
- 25 µL / determination
- standards:
- 6 calibrators, ready to use
- Standard range:
- 0 / 0.77 - 123 ng/mL
- storage:
- 2 - 8 °C
- sensitivity:
- Please see protocol for assay-specific performance characteristics
- Species:
- Human
