Glucagon is a peptide hormone with a molecular weight of 3,485 consisting of 29 amino acid residues, secreted by the alpha cells of the pancreas.
It acts on the liver to promote the production and release of glucose through glycogenolysis and glycogenesis, thereby raising blood glucose.
It has been indicated that dysregulation of glucagon secretion is important in the pathogenesis of diabetes as it is involved with insulin in the regulation of glucose homeostasis.
Proglucagon, a precursor of glucagon produces similar peptides such as glicentin and oxyindomodulin, which have amino acid sequences that overlap with glucagon by different processing process depending on the produced cell.
It is required to use the assay system which specifically detects glucagon and does not cross reacts to similar peptides for accurately evaluating glucagon in blood.
This ELISA kit can specifically measure mouse glucagon produced in α-cells of pancreas by combining antibodies that recognize both the C-terminus and the N-terminus.
For research use only. Not for use in diagnostic procedures.
- Assay Description:
- 60 minutes (RT) + Overnight incubation (4°C) + 60 min. (4°C) + 30 min. (RT) = Overnight + 2 hr. 30 min. total incubation time
- Catalog number:
- 27822
- Configuration:
- 96 Determinations, 12x8 removable strips
- Controls:
- None provided
- Design:
- Sandwich ELISA
- FDA Status:
- For research use only, not for use in diagnostic procedures
- Protocol:
- Notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- MSDS:
- Please Inquire
- Sample Types:
- Mouse EDTA-plasma
- Sample Note:
- EDTA-plasma samples should be collected under chilled conditions (on ice). It is recommended that aprotinin be pre-added to blood collection tubes to achieve a final concentration of 500 KIU/mL.
- Sample Volume:
- 100 μL of properly diluted unknown / determination
- Standards:
- 7 standards, serially diluted from 1 prepared lyophilized standard
- Standard Range:
- 0 / 0.13 - 8 pmol/L
- Sensitivity:
- 0.049 pmol/L
- Storage:
- 2 - 8 °C
- Species:
- Mouse
