Solid phase sandwich ELISA for the determination of mouse soluble Alpha Klotho (α-Klotho) in mouse urine. For research use only, not for use in diagnostic procedures. <br><br>
α-klotho (Alpha Klotho) was identified as an extremely down-regulated gene in the genetically-modified mouse of which phenotype is very similar to various symptoms of human aging. Then, the sequences of Alpha Klotho genes have been identified in various species including humans based on the one of mouse. Alpha Klotho protein is a 130 kDa, one-transmembrane protein and its expression is confirmed in the kidneys and the parathyroid glands. In recent years, it has become clear that Alpha Klotho is an important molecule within a living organism regulating the metabolism of mineral such as calcium and phosphorus. Therefore, it is considered that, in Alpha Klotho mouse, early aging-like symptoms are induced by impaired mineral homeostasis caused by decreased expression of Alpha Klotho. Meanwhile, it is reported that the long N-terminal extracellular domain which comprises the major portion of sequence of Alpha Klotho protein is released free into blood by shedding. However, there are many unclear points about functions and changes in concentration of free (soluble) Alpha Klotho protein, so it has been required to develop the measurement system of Alpha Klotho. This ELISA kit can measure mouse soluble Alpha Klotho protein in mouse urine. <br><br>
Control set 27601-QC is available for purchase.<br><br>
Keywords:
α-Klotho / Alpha Klotho / FGF23 / Kidney disease / Chronic Kidney disease (CKD) / Mineral Bone Disease (MBD) / Osteoporosis / Aging / Alzheimer's Disease
- Assay Description:
- 1 hour incubation (37°C) + 30 min. (2 - 8°C) + 30 min. (RT) = 2 hours total incubation time
- Catalog number:
- 27601
- configuration:
- 96 Determinations, 12x8 removable strips
- design:
- Solid phase sandwich enzyme-linked immunosorbent assay (ELISA)
- MSDS:
- FDA Status:
- For research use only, not for use in diagnostic procedures
- Protocol:
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- controls:
- 27601-QC
- Sample types:
- Mouse urine
- Sample volume:
- 100 µL of properly diluted unknown / determination
- standards:
- 7 standards, serially diluted from 1 prepared lyophilized standard
- Standard range:
- 78.13 - 5000.0 pg/mL
- storage:
- 2 - 8 °C
- sensitivity:
- 18.06 pg/mL
- Species:
- Mouse
- Sample Information:
- Additional Info:
