27998
Assay Description
1 hour incubation (RT) + 30 min. (RT) + 30 min. (RT) = 2 hours total incubation time
Catalog number
27998
configuration
96 Determinations, 12x8 removable strips
Notes
The protocol for the Alpha Klotho product (see above) is intended to serve as an example only. Please refer to the instructions for use provided with the assay kit for precise details.
design
Solid phase sandwich ELISA using 2 kinds of highly specific antibodies
FDA Status
For research use only, not for use in diagnostic procedures
Sample types
Serum, plasma (EDTA, Heparin, Citrate), urine and cell culture supernatant
Sample volume
100 µL of properly diluted unknown / determination
Standards
8 standards, serially diluted from 1 prepared lyophilized standard
Standard range
0 / 93.75 - 6000 pg/mL
storage
2 - 8 °C
Sensitivity
6.15 pg/mL
Species
Human
27601
Assay Description
1 hour incubation (37°C) + 30 min. (2 - 8°C) + 30 min. (RT) = 2 hours total incubation time
Catalog number
27601
configuration
96 Determinations, 12x8 removable strips
design
Solid phase sandwich enzyme-linked immunosorbent assay (ELISA)
FDA Status
For research use only, not for use in diagnostic procedures
notes
The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
Sample types
Mouse urine
Sample volume
100 µL of properly diluted unknown / determination
standards
7 standards, serially diluted from 1 prepared lyophilized standard
Standard range
78.13 - 5000.0 pg/mL
storage
2 - 8 °C
sensitivity
18.06 pg/mL
Species
Mouse
27901
Assay Description
Overnight (ON) incubation (2-8℃) + 60 min. (2-8℃) + 30 min. (RT) = ON + 1 hour 30 min. total incubation time
Catalog number
27901
configuration
96 Determinations, 12x8 removable strips
Controls
None provided
design
Solid phase sandwich ELISA using 2 highly specific antibodies
FDA Status
For research use only, not for use in diagnostic procedures
MSDS
Please Inquire
Notes
The protocol for the secreted Alpha Klotho product (see above) is intended to serve as an example only. Please refer to the instructions for use provided with the assay kit for precise details.
Sample types
Serum, EDTA-plasma, and cerebrospinal fluid (CSF)
Sample volume
100 µL of properly diluted unknown / determination
Standards
8 standards, serially diluted from 1 prepared lyophilized standard
Standard range
0 / 70.31 - 4500 pg/mL
storage
2 - 8 °C
Sensitivity
9.87 pg/mL
Species
Human
27194
Assay Description
Overnight incubation (4°C) + 30 min. (4°C) + 30 min. (RT) = Overnight + 1 hour total incubation time
Catalog number
27194
configuration
96 Determinations, 12x8 removable strips
controls
None provided
design
Solid phase sandwich ELISA using 2 kinds of high specific antibodies
FDA Status
For research use only, not for use in diagnostic procedures
notes
The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
Sample types
Cell culture supernatant
Sample volume
100 μL of properly diluted unknown / determination
standards
8 standards, serially diluted from 1 prepared lyophilized standard
Standard range
0 / 56.25 - 3600 pg/mL
storage
2 - 8 °C
sensitivity
10.55 pg/mL
Species
Rat
Products related
The control set is available for sale on request
IBCRA3002
Catalog number
IBCRA3002
concentration
1 mg/ml (determined by Bradford assay)
formulation
Liquid. In 20 mM Tris-HCl buffer (pH 7.5) containing 50 mM NaCl, 1 mM EDTA
molecular_weight
20.1kDa (175aa)
notes
For research use only, not for use in diagnostic procedures.
The datasheet for this product (see above) is intended to serve as an example only. Please refer to the datasheet provided with the protein for precise details.
Other names
CRYA2, HSPB5
purity
> 95 % by SDS €“ PAGE
size
100 µg
storage
Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.