The Human Secretoneurin ELISA is a sandwich Enzyme-linked Immunoassay (ELISA). Two secretoneurin-specific sheep monoclonal antibodies (MABs) are used in the assay. One MAB is biotinylated and the second is HRP-conjugated. The biotinylated antibody is added to the wells of a streptavidin coated microtiter plate. After washing, diluted calibrators, controls and samples are added, and secretoneurin present is bound to the immobilized antibody. After incubation of samples the wells are washedto remove unbound sample material, and the HRP-conjugated antibody is added. After incubation and removal of unbound conjugate by washing, the TMB substrate is added. The blue color developed is directly proportional to the amount of secretoneurin present in the calibrators, controls, and samples. The stop solution changes the color from blue to yellow, and the intensity of the color is measured in a microtiter plate reader.
For research use only, not for use in diagnostic procedures.
- Assay Description:
- Test
- Catalog number:
- 47-11
- Configuration:
- 96 Determinations, 12x8 removable strips
- Controls:
- 2 controls, ready to use
- Design:
- Enzyme immunoassay (ELISA) technique
- FDA Status:
- For research use only, not for use in diagnostic procedures
- MSDS:
- Please Inquire
- Protocol:
- Notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Sample types:
- Serum and Plasma
- Sample volume:
- 100μL of 1:10 diluted sample
- Standard:
- 6 calibrators, ready to use
- Standard range:
- 0 / 10 - 250 pmol/L
- Storage:
- 2 - 8 °C
- Sensitivity:
- 7.6 pmol/L
- Pamphlet:
- Publication:
