Enzyme immunoassay (ELISA) for the determination of S100B concentrations in serum, plasma, cell culture supernatant and other biological samples. For research use only, not for use in diagnostic procedures.
BACKGROUND: Uniprot ID： P04271
S100B belongs to the S100 subgroup of the EF-hand family of Ca2+ binding proteins. It is a homodimer that is expressed primarily in the brain by astrocytes, oligodendrocytes and Schwann cells. S100B has multiple intracellular functions but can also be secreted from cells to exert extracellular functions. Some of the extracellular functions of S100B may be mediated by RAGE (receptor for advanced glycation end products). Blood levels of S100B can be used to monitor the extent of brain injury and malignant melanoma.
- Assay Description:
- 90 min incubation (37C) + 60 min (37C) + 30 min. (37C) + 10-20 min. (37C) = 3 hours, 20 min. total incubation time
- Catalog number:
- IB08251
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- None provided
- design:
- Enzyme immunoassay (ELISA) technique
- FDA Status:
- For research use only, not for use in diagnostic procedures
- Protocol:
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- MSDS:
- Available Upon Request
- Sample types:
- Serum, Plasma, and other biological samples
- Sample volume:
- 100 μL properly diluted sample / determination
- standards:
- 8 levels from one lyophilized stock
- Standard range:
- 0 - 2000 pg/L
- storage:
- 2 - 8 °C
- Species:
- Human
