Enzyme immunoassay for detection of OC/BGP in serum, plasma, tissue homogenates and other biological fluids.
Osteocalcin (OCa), which is otherwise known as bone gla-protein (BGP) is the most abundant noncollagenous protein in bone matrix, and a small fraction is released into the circulation after synthesis by osteoblasts. The only other cells to express OCa protein are odontoblasts and hypertrophic chondrocytes, so OCa has tissue specificity, and it has been widely used as a sensitive and specific marker of osteoblast function in human research studies.
- Assay Description:
- 90 min. incubation (37°C) + 60 min. (37°C) + 10-20 min. (37°C) = 2 hours, 50 min. total incubation time
- Catalog number:
- IB08033
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- None provided
- design:
- Enzyme immunoassay (ELISA) technique
- FDA Status:
- For research use only, not for use in diagnostic procedures
- Protocol:
- Sample types:
- Serum, plasma, tissue homogenates and other biological fluids
- Sample volume:
- 100μl of properly diluted sample
- standards:
- 7 standards, from 1 vial of lyophilized stock
- Standard range:
- 0 / 0.234 – 15 ng/mL
- sensitivity:
- 0.141 ng/mL
- Species:
- Human
- storage:
- 4 °C
