Solid phase sandwich ELISA for the determination of human Fibroblast Growth Factor 23 (FGF23) in human serum or EDTA plasma. For research use only, not for use in diagnostic procedures.
The kit can measure both intact FGF23 and FGF23 contained in the C-terminal fragment.
FGF23 is a hormone that lowers serum phosphate levels by acting on the Klotho–FGFR complex, inhibiting phosphate reabsorption in the renal proximal tubules and suppressing intestinal phosphate absorption by reducing circulating levels of active vitamin D.
In chronic kidney disease (CKD), FGF23 levels are known to increase as kidney function declines to prevent phosphate accumulation. As CKD progresses, the ability of FGF23 and parathyroid hormone (PTH) to suppress phosphate reabsorption reaches its limit, thus increasing the risk of hyperphosphatemia and secondary hyperparathyroidism. Therefore, measuring FGF23 levels is important for studying CKD and secondary hyperparathyroidism. It is also considered useful for researching bone-related disorders such as hypophosphatemic rickets and osteomalacia.
In chronic kidney disease (CKD), FGF23 levels are known to increase as kidney function declines to prevent phosphate accumulation. As CKD progresses, the ability of FGF23 and parathyroid hormone (PTH) to suppress phosphate reabsorption reaches its limit, thus increasing the risk of hyperphosphatemia and secondary hyperparathyroidism. Therefore, measuring FGF23 levels is important for studying CKD and secondary hyperparathyroidism. It is also considered useful for researching bone-related disorders such as hypophosphatemic rickets and osteomalacia.
- Assay Description:
- 60 min incubation (37°C) + 30 min. (2-8°C) + 30 min. (RT) = 2 hour total incubation time
- Catalogue number:
- 27266
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- None provided
- design:
- Solid phase sandwich ELISA
- FDA Status:
- For research use only, not for use in diagnostic procedures
- MSDS:
- Available Upon Request
- Protocol:
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Sample types:
- Human Serum or EDTA plasma
- Sample volume:
- 100 µL of properly diluted unknown / determination
- standards:
- 8 standards, serially diluted from 1 prepared lyophilized standard
- Standard range:
- 0 / 15.63 - 1,000 pg/mL
- storage:
- 2 - 8 °C
- sensitivity:
- 3.63 pg/mL
- Species:
- Human
- Pamphlet:
