Sandwich enzyme-linked immune-sorbent assay (ELISA) for the determination of human HFABP in serum, cell culture supernatants or tissue homogenate. For research use only, not for use in diagnostic procedures.
- Assay Description:
- 90 min. incubation (37°C) + 60 min. (37°C) + 30 min. (37°C) = 3 hours total incubation time
- Catalog number:
- BE69228
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- None provided
- design:
- Sandwich enzyme-linked immune-sorbent assay (ELISA) technique
- FDA Status:
- For research use only, not for use in diagnostic procedures
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Other names:
- Heart-type fatty acid binding protein (hFABP), mammary-derived growth inhibitor
- Protocol:
- Sample types:
- Serum, cell culture supernatants or tissue homogenate
- Sample volume:
- 100 µL of properly diluted unknown / determination
- standards:
- 8 standards, serially diluted from 1 prepared lyophilized standard
- Standard range:
- 0 / 15.6 - 1000.0 pg/mL
- storage:
- Store at 4°C for 6 months, or at -20°C for one year. Store HRP conjugated antibody at 4°C for one year
- sensitivity:
- 1 pg/mL
- Species:
- Human