Product Spotlight | Prorenin Receptor Protein ELISA
Posted by Brandon Savela on Mar 7th 2018
(Pro)renin receptor/(P)RR is a common receptor protein between renin and prorenin. It has an ability equivalent to renin to bind angiotensinogen and a catalytic activity for conversion from angiotensinogen to angiotensin I once it binds to the (P)RR. Additionally, its intracellular signal transmission promotes once the (P)RR is stimulated by binding with prorenin. From the fact, it is believed that (P)RR research is important for development of strategy for a new remedy which enable to suppress over activated tissue RAA (Renin-Angiotensin-Aldosterone) system. (P)RR is a 39 kDa, single transmembrane receptor protein and it is reported that it generates 29 kDa soluble form cleaved by furin. Thus the quantitative assay of soluble (P)RR in blood or urine samples is expected to contribute a new perception for clarification of disease mechanisms and development of new tools for diseases. This assay kit is enabled to measure the concentration of soluble (Pro)renin receptor in human, mouse and rat blood or urine samples.
Soluble (Pro)renin Receptor ELISA
| Catalog Number | 27782 |
|---|---|
| Species | Human |
| Design | Solid phase sandwich ELISA using 2 kinds of highly specific antibodies. |
| Standards | 8 standards, serially diluted from 1 prepared lyophilized standard. |
| Controls | None provided. |
| Sample Types |
Human: serum, EDTA-plasma, urine. Mouse and Rat: serum, EDTA-plasma. Cell culture supernatant. |
| Sample Volume | 100 μL of properly diluted unknown / determination. |
| Assay Desc. | Overnight incubation (4°C) + 1 hour (4°C) + 30 min. (RT) = Overnight + 1 hour, 30 min. total incubation time. |
| Standard Range | 0 / 125 - 8000 pg/mL |
| Sensitivity | 24 pg/mL |
| Storage | 2 - 8 °C |
| Configuration | 96 Determinations, 12x8 removable strips |
| FDA Status | For research use only, not for use in diagnostic procedures. |
