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Amyloid Beta (N3pE-40) Aβ

$583.00
SKU:
27418
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Solid phase sandwich ELISA for the determination of Human Aβ (N3pE-40) in brain tissue extract. For research use only, not for use in diagnostic procedures.<br><br>

The first case of Alzheimer's disease was defined and reported in 1907 by the German scientist, Dr. A. Alzheimer. His studies have shown that this is the main cause of dementia in the elderly. The plaques which appear in the brains of individuals who suffer AD are mostly constituted by the Amyloid β protein (Aβ). Aβ is a peptide which consists of 40 or 42 (43) amino acids, and reports show that this is cleaved by β- and γ-secretase from the amyloid precursor protein. APP is a trans-membrane protein consisting of 695, 751, or 770 amino acids. Reports have shown many variants of Aβ exist and are clarified into the culture supernatant from the APP cDNA transfected mouse neuroblastoma cell. Furthermore, in 1995, a dominant and differential deposition of distinct β amyloid peptide species, Aβ (N3pE), in senile plaques was found by Saido et al. This modified molecule, starting at the 3rd amino terminal residue, glutamate, was discovered to convert to pyroglutamate through intramolecular dehydration. This kit can measure human Aβ (N3pE-40). For measuring Human Aβ (N3pE-42), use IBL Code No.27716, Human Amyloid β (N3pE-42) Assay Kit - IBL. IBL has many other kinds of Amyloidβ-related products for AD research. They are very specific assay systems for each target and they can be used according to the purpose of study.<br><br>

Keywords:
Alzheimer's Disease / Amyloidβ / Amyloid-β / Amyloid Beta / Aβ / Beta Amyloid

Assay Description:
Overnight Incubation (4°C) + 1 hour (4°C) + 30 min. (RT) = Overnight + 1 hour, 30 min. total incubation time
Catalog number:
27418
configuration:
96 Determinations, 12x8 removable strips
controls:
None provided
design:
Solid phase sandwich ELISA using 2 kinds of high specific antibodies
FDA Status:
For research use only, not for use in diagnostic procedures
MSDS:

MSDS

notes:
The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
Protocol:

Protocol

Sample types:
Brain tissue extract
Sample volume:
100 μL of properly diluted unknown / determination
standards:
8 standards, serially diluted from 1 prepared lyophilized standard
Standard range:
0 / 3.13 - 200 pg/mL
storage:
2 - 8 °C
sensitivity:
0.31 pg/mL
Species:
Human
Additional Info:

AD Assays and Antibodies

Comprehensive Lineup

References:

References