This kit is a solid phase sandwich ELISA (Enzyme-linked Immunosorbent Assay). As a primary antibody is coated on a plate, samples and standard are added into the wells for the 1st reaction. After the reaction, HRP-conjugated secondary antibody is added into the wells for the 2nd reaction. After a washing step is performed to wash away any unbound secondary antibody, Tetra Methyl Benzidine (TMB) is added to the wells and color develops.
This product is for research use only. It is not intended for in-vitro diagnostic use.
Commonly Asked Question for Testing with the 27820 CLEC-2 ELISA : 27820-FAQ
- Assay Description:
- 1 hour incubation (37°C) + 1 hour incubation (2-8°C) + 30 minute incubation (R.T.) = 2 hr 30 min. incubation time
- Catalog number:
- 27820
- Configuration:
- 96 Determinations, 12x8 removable strips
- Controls:
- None provided
- Design:
- Solid phase sandwich ELISA
- FDA Status:
- For research use only, not for use in diagnostic procedures
- Sample Size:
- 100 uL of properly diluted sample
- MSDS:
- Available upon request
- Sample types:
- Citrated Plasma
- Standard Range:
- 0 / 6.25 - 400 pg/mL
- Storage:
- 2 - 8 °C
- Species:
- Human
- Sample Storage:
- Recommend storing samples at -40 °C or below immediately after plasma seperation.
- Protocol:
- Notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Pamphlet:
- FAQ:
