α-MSH in standards and samples compete with 125I-labelled α-MSH in binding to the antibodies. 125I-α-MSH binds in a reverse proportion to the concentration of α-MSH in standards and samples.
In order to increase the sensitivity of the assay a sequential assay with delayed addition of 125I-α-MSH is performed. Antibody bound 125I-α-MSH is separated from the free fraction using the double antibody polyethylene glycol precipitation technique.
The radioactivity of the precipitates is measured. The antiserum used in this kit is directed to the C-terminal part of the α-MSH molecule and shows no cross-reactivity with adrenocorticotropic hormone.
Radioimmunoassay (RIA) for the determination α-melanocyte stimulating hormone (α-MSH) in human plasma or cerebrospinal fluid. For research use only, not for use in diagnostic procedures.
- Assay Description:
- 20 hour incubation (2-8°C) + 20 hours (2-8°C) + 30 min. (2-8°C) + Centrifuge 15 min. (4°C) = 40 hours, 45 min. total incubation time
- Catalog number:
- RB303RUO
- configuration:
- Reagents for 100 determinations
- controls:
- 2 controls, lyophilized
- design:
- Radioimmunoassay (RIA) technique
- FDA Status:
- For research use only, not for use in diagnostic procedures
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Protocol:
- Other names:
- α-Melanocyte Stimulating Hormone, Alpha-MSH
- Sample types:
- Human plasma or cerebrospinal fluid
- Sample volume:
- 100 µL / determination
- standards:
- 7 standards, serially diluted from 1 prepared lyophilized standard
- Standard range:
- 0 / 4.7 - 150 pmol/L
- storage:
- 2 - 8 °C
- sensitivity:
- 3 pmol/L
- Species:
- Human
- radioactivity:
- 0.75 µCi (28 kBq)
