Exposure to tobacco smoke can be detected by measuring nicotine and its metabolites. Nicotine has a short half life and is not used as a marker for tobacco smoke exposure. Cotinine due to its longer half life has been used in research as a reliable marker for smoking status and smoking cessation studies. This product detects cotinine in rodent samples. This assay is intended for research in rodent models to study nicotine metabolism, pharmacokinetics, and the biological impact of environmental tobacco smoke.
The Cotinine kit is a solid phase competitive ELISA. The samples, Cotinine antibody reagent, and Cotinine enzyme conjugate are added to the wells coated with Goat anti-rabbit IgG. Cotinine in the samples competes with a Cotinine enzyme (HRP) conjugate for binding sites on the antibody reagent which is then absorbed onto the plate. Unbound Cotinine and Cotinine enzyme conjugate is washed off by washing step. Upon the addition of the substrate, the intensity of color is inversely proportional to the concentration of Cotinine in the samples. A standard curve is prepared relating color intensity to the concentration of the Cotinine.
For Research Use Only. For professional use only. Not for use in diagnostic procedures.
- Assay Description:
- 1 hour incubation (RT) + 30 min. (RT) = 1 hour, 30 min. total incubation time
- Catalog number:
- IB19138
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- 2 controls, ready to use
- design:
- Enzyme immunoassay (ELISA) technique
- FDA Status:
- For research use only, not for use in diagnostic procedures
- Protocol:
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Sample types:
- Serum
- Sample volume:
- 10 μL / determination
- standards:
- 6 calibrators, ready to use
- Standard range:
- 0 / 0.6 - 50.0 ng/mL
- storage:
- 2 - 8°C
- Species:
- Mouse or Rat
