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Sandwich enzyme-linked immunosorbent assay (ELISA) for the determination of intact proinsulin in mouse and rat EDTA-plasma, serum and cell culture supernatant. For research use only, not for use in diagnostic procedures.<br><br>
Proinsulin is a precursor of insulin that is synthesized in the β cells of the islet of Langerhans of pancreas.<br><br>
Most of the proinsulin synthesized in the rough endoplasmic reticulum of β cells is stored in the secretory granules. Proinsulin is cleaved by prohormone converting enzyme (PC) 1/3 at the junction of the C-peptide with the B-chain of insulin, subsequently it is cleaved by PC2 at the junction of the C-peptide with the A-chain of insulin, consequently it is divided into insulin that consists of 21 amino-acid A chain and 30 amino-acid β chain and c-peptide that consists of 33 amino acid.<br><br>
It has been considered that the conversion rate from proinsulin to insulin in type 2 diabetes patients decreases due to dropping down of PC1/3 enzymatic activity by abnormal function of pancreatic β cells. Thus, intact proinsulin level in blood or the ratio of intact proinsulin and insulin (P/I ratio) can be considered to become a potential biomarker that reflects functional change of the PC1/3 activity at pancreaticβ cell.<br><br>
Especially, It has been known that the P/I ratio is a valuable pancreatic β cell evaluation method that does not require a loading test as it can be measured using blood samples in fasting.<br><br>
This ELISA kit can be used for measuring intact proinsulin, not including cleaved intermediate products, in mouse and rat samples.
Sandwich enzyme-linked immunosorbent assay (ELISA) for the determination of intact proinsulin in mouse and rat EDTA-plasma, serum and cell culture supernatant. For research use only, not for use in diagnostic procedures.<br><br>
Proinsulin is a precursor of insulin that is synthesized in the β cells of the islet of Langerhans of pancreas.<br><br>
Most of the proinsulin synthesized in the rough endoplasmic reticulum of β cells is stored in the secretory granules. Proinsulin is cleaved by prohormone converting enzyme (PC) 1/3 at the junction of the C-peptide with the B-chain of insulin, subsequently it is cleaved by PC2 at the junction of the C-peptide with the A-chain of insulin, consequently it is divided into insulin that consists of 21 amino-acid A chain and 30 amino-acid β chain and c-peptide that consists of 33 amino acid.<br><br>
It has been considered that the conversion rate from proinsulin to insulin in type 2 diabetes patients decreases due to dropping down of PC1/3 enzymatic activity by abnormal function of pancreatic β cells. Thus, intact proinsulin level in blood or the ratio of intact proinsulin and insulin (P/I ratio) can be considered to become a potential biomarker that reflects functional change of the PC1/3 activity at pancreaticβ cell.<br><br>
Especially, It has been known that the P/I ratio is a valuable pancreatic β cell evaluation method that does not require a loading test as it can be measured using blood samples in fasting.<br><br>
This ELISA kit can be used for measuring intact proinsulin, not including cleaved intermediate products, in mouse and rat samples.
