Enzyme immunoassay for detection of Free Homocysteine (HCY) concentrations in serum, plasma, cell culture supernatant and other biological samples. For research use only. Not for use in diagnostic procedures.
Homocysteine (HCY) is a sulfur-containing amino acid, which is the product of demethylation of methionine. More than 80% of HCY in serum or plasma exists in the form of bound or free dimer (HCY-proteins, SAH, HCY-CYS, HCY-HCY, etc.). HCY has no antigenicity. S-adenosine homocysteine hydrolase (in Antibody Dilution Buffer) was used to generate Ado HCY for detection of Free HCY content indirectly.
- Assay Description:
- 45 min. incubation (37°C) + 30 min. (37°C) + 10-20 min. (37°C) = 1 hour, 35min. total incubation time
- Catalog number:
- IB08058
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- None provided
- design:
- Enzyme immunoassay (ELISA) technique
- FDA Status:
- For research use only, not for use in diagnostic procedures
- Protocol:
- Notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Sample types:
- Serum, plasma, cell culture supernatant, and other biological samples
- Sample volume:
- 50 μL of properly diluted sample
- standards:
- 8 standards, from 1 vial of lyophilized stock
- Standard range:
- 0 / 0.25 - 16 umol/L
- sensitivity:
- 0.15 umol/L
- storage:
- 2-8 °C
- Species:
- Human (Can be used for other mammalian samples)
