Enzyme immunoassay for determination of Corticosteroid-binding Globulin (CBG) (SERPINA6) concentrations in serum, plasma,
cell culture supernatant and other biological samples. For research use only. Not for use in diagnostic procedures.<br><br>
SerpinA6, also known as CBG (Corticosterone-Binding Globulin) or Transcortin, is a 405 amino acid α-globulin secreted protein that belongs to the serpin family. Synthesized in the liver and present in glycocorticoid responsive cells, SerpinA6 functions as the primary transport protein for progestins and glucorticoids within the blood. Additionally, SerpinA6 has corticosteroid-binding properties through which it can regulate the physiological binding of serum cortisol within the cell. Defects in the gene encoding SerpinA6 are the cause of corticosteroid-binding globulin deficiency (CBG deficiency), a rare disorder characterized by reduced corticosteroid-binding rates that result in hypo/hypertension and muscle fatigue.
- Assay Description:
- 90 min. incubation (37°C) + 60 min. (37°C) + 60 min. (37°C) + 10-20 min. (37°C) = 3 hour, 50 min. total incubation time
- Catalog number:
- IB08059
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- None provided
- design:
- Enzyme immunoassay (ELISA) technique
- FDA Status:
- For research use only, not for use in diagnostic procedures
- Protocol:
- 0
- Notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Sample types:
- Serum, plasma, cell culture supernatant, and other biological samples
- Sample volume:
- 100 μL of properly diluted sample
- standards:
- 8 standards, from 1 vial of lyophilized stock
- Standard range:
- 0 / 15.625 - 1000 ng/mL
- sensitivity:
- 9.375 ng/mL
- storage:
- 2-8 °C
- Species:
- Human
