Solid phase sandwich ELISA for the determination of Human ApoB-100 in serum, EDTA-plasma and cell culture media. For research use only, not for use in diagnostic procedures.
Though the lipids (such as fat and cholesterol) are not soluble in water, apolipoproteins are carrier proteins that combine with lipids to form lipoprotein particles which are water-soluble and can be carried through water-based circulation (i.e., blood, lymph). Apolipoproteins are classified according to their forms and functions, and there are six major classes and several sub-classes. ApoB (ApolipoproteinB) is the primary apolipoprotein of LDL and is considered to reflect change of LDL level in blood. There are two types apolipoprotein in ApoB. One is ApoB-100 which correlates with cholesterol level well and is released as LDL and VLDL. Another is ApoB-48 which presents being incorporated into chylomicrons and correlates well with tryglyceride. Generally, the concentration of ApoB in blood has been measured as the total of ApoB-100 and ApoB-48. This kit can measure only ApoB-100 in plasma samples using ApoB-100 specific antibodies which don’t recognize ApoB-48. This highly sensitive sandwich ELISA can also measure ApoB-100 in cell culture media or various fractionated lipoprotein samples.
- Assay Description:
- 60 min. incubation (37°C) + 30 min. (37°C) + 30 min. (RT) = 2 hours total incubation time
- Catalogue number:
- 27181
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- None provided
- design:
- Solid phase sandwich ELISA using 2 kinds of high specific antibodies.
- FDA Status:
- For research use only, not for use in diagnostic procedures
- MSDS:
- Protocol:
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Sample types:
- Serum, EDTA-plasma, Cell culture supernatant
- Sample volume:
- 100 µL of properly prepared unknown / determination
- standards:
- 8 standards, serially diluted from 1 prepared lyophilized standard
- Standard range:
- 0.13 - 8.4 µg/mL
- storage:
- 2 - 8 °C
- sensitivity:
- 0.03 µg/mL
- Species:
- Human
- Additional Info:
- References:
