Enzyme immunoassay (ELISA) for the direct determination of 5α-Androstane-3α, 17β diol Glucuronide (3α Diol G) in human serum.
The 3α-Diol G ELISA is a competitive immunoassay. Competition occurs between 3α-Diol G present in calibrators, controls, samples and an enzyme-labelled antigen (HRP conjugate) for a limited number of anti-3α-Diol G antibody binding sites on the microplate wells. After a washing step that removes unbound materials, the TMB substrate (enzyme substrate) is added which reacts with HRP to form a blue-coloured product that is inversely proportional to the amount of 3α-Diol G present. Following an incubation, the enzymatic reaction is terminated by the addition of the stopping solution, converting the colour from blue to yellow. The absorbance is measured on a microplate reader at 450 nm. A set of calibrators is used to plot a calibrator curve from which the amount of 3α-Diol G in samples and controls can be directly read.
For research use only, not for use in diagnostic procedures.
Click for IFU - 3α-Diol G ELISA IB59102
- Assay Description:
- 30 min. (RT) incubation + 15 min. (RT) = 45 min. total incubation time
- Catalog number:
- IB59102
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- 2 controls, ready to use
- design:
- Enzyme immunoassay (ELISA) technique
- FDA Status:
- For research use only, not for use in diagnostic procedures
- MSDS:
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Protocol:
- Sample types:
- Human serum
- Sample volume:
- 50 μL / determination
- standards:
- 6 calibrators, ready to use
- Standard range:
- 0 / 0.25 - 50 ng/mL
- storage:
- 2 - 8 °C
- sensitivity:
- Please see protocol for assay-specific performance characteristics
- Species:
- Human
