Thyroglobulin

Thyroglobulin is an indirect solid phase enzyme immunometric assay (ELISA). It is designed for the quantitative measurement of human thyroglobulin in serum or plasma. The assay is based on microplates coated with highly specific anti-human-thyroglobulin antibodies.<br><br>

Thyroglobulin present in a sample binds to the antibody coated forming an antigen-antibody-complex. Washing of the microwells removes unbound unspecific serum and plasma components. During incubation with enzyme-conjugate immunologically a conjugate/antibody/antigen complex is formed. Washing of the microwells removes unbound conjugate. An anti-thyroglobulin enzyme substrate in the presence of bound conjugate hydrolyzes to form a blue color. The addition of an acid stops the reaction forming a yellow end-product. The intensity of this yellow color is measured photometrically at 450nm. The amount of color is directly proportional to the concentration of antibodies present in the original sample. This assay includes a recovery test. For research use only, not for use in diagnostic procedures.