Nampt (Visfatin/PBEF) (Intracellular) ELISA, IB99528 (RUO)

Nampt (Visfatin/PBEF) (Intracellular)

$859.00

Current Stock:

SKU:

IB99528

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Overview
Description

Assay Description:: 1 hour incubation (37°C) + 1 hour (37°C) + 1 hour (37°C) + 10 min. (RT) = 3 hours, 10 min. total incubation time
Catalog number:: IB99528
configuration:: 96 Determinations, 6x16 removable strips
controls:: None provided
design:: Sandwich enzyme linked-immunosorbent assay (ELISA) technique
FDA Status:: For research use only, not for use in diagnostic procedures
MSDS:: MSDS
notes:: The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
Protocol:: Protocol
Sample types:: Cell lysates or cell-based assays (screening)
Sample volume:: 100 µL of properly diluted unknown / determination
standards:: 8 standards, serially diluted from 1 prepared lyophilized standard
Standard range:: 0 / 0.25 - 16 ng/mL
storage:: 2 - 8 °C
sensitivity:: 30 pg/mL
Species:: Human

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application

ELISA, WB.

Catalog number

IBAVI0816

clone

4D5

concentration

1mg/ml

Datasheet

Click for Datasheet

formulation

Liquid. In Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% Glycerol.

immunogen

Recombinant human Visfatin (1-491aa) purified from E. coli

isotype

Mouse IgG1heavy chain and kappa light chain

notes

For research use only, not for use in diagnostic procedures.
The datasheet for this product (see above) is intended to serve as an example only. Please refer to the datasheet provided with the antibody for precise details.

Other names

Please see datasheet.

size

100 µL

storage

4°C. For long term storage, aliquot and store at -20°C.

Species

Human

Host

Mouse
Visfatin, 1-491aa, Human, His-tagged, Recombinant, E.coli

IBVST0801

$472.00

Catalog number

IBVST0801

concentration

1 mg/ml (determined by Bradford assay)

Datasheet

Click for Datasheet

formulation

Liquid in 20mM Tris pH 8.0, 0.1mM DTT, 10%glycerol

molecular_weight

57 kDa ( 511 aa), confirmed by MALDI-TOF.

notes

For research use only, not for use in diagnostic procedures.
The datasheet for this product (see above) is intended to serve as an example only. Please refer to the datasheet provided with the protein for precise details.

Other names

Pre-B-cell colony-enhancing factor 1, NAMPT, MGC117256, Nicotinamide phosphoribosyltransferase precursor, PBEF, PBEF1, VF, NAMPT, Visfatin, Nicotinamide phosphoribosyltransferase precursor, pre-B-cell colony enhancing factor 1,

purity

> 95 % by SDS - PAGE

size

100 µg

storage

Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Sirtuin 1 (Intracellular)

IB99522

$776.00

Assay Description

1 hour incubation (37°C) + 1 hour (37°C) + 1 hour (37°C) + 10 min. (RT) = 3 hours, 10 min. total incubation time.

Catalog number

IB99522

configuration

96 Determinations, 6x16 removable strips

controls

None provided.

design

Sandwich enzyme linked-immunosorbent assay (ELISA) technique.

FDA Status

For research use only, not for use in diagnostic procedures.

MSDS

Click for MSDS

notes

The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.

Protocol

Click for Protocol

Sample types

Cell lysates or cell-based assays (screening).

Sample volume

100 µL of properly diluted unknown / determination.

standards

8 standards, serially diluted from 1 prepared lyophilized standard.

Standard range

0 / 0.03125 - 2 ng/mL

storage

2 - 8 °C

sensitivity

30 pg/mL

Species

Human
Nampt (Visfatin/PBEF)

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Assay Description

Overnight incubation (4°C) + 1 hour (37°C) + 1 hour (37°C) + 10 min. (RT) = Overnight + 2 hours, 10 min. total incubation time

Catalog number

IB99526

configuration

96 Determinations, 6x16 removable strips

controls

None provided

design

Sandwich enzyme linked-immunosorbent assay (ELISA) technique

FDA Status

For research use only, not for use in diagnostic procedures

MSDS

MSDS

notes

The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.

Protocol

Protocol

Sample types

Serum

Sample volume

100 µL of properly diluted unknown / determination

standards

8 standards, serially diluted from 1 prepared lyophilized standard

Standard range

0 / 0.125 - 8 ng/mL

storage

2 - 8 °C

sensitivity

30 pg/mL

Species

Human
Nampt (Visfatin/PBEF) (Mouse/Rat)

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Assay Description

Overnight incubation (4°C) + 1 hour (37°C) + 1 hour (37°C) + 10 min. (RT) = Overnight + 2 hours, 10 min. total incubation time

Catalog number

IB99527

configuration

96 Determinations, 6x16 removable strips

controls

None provided

design

Sandwich enzyme linked-immunosorbent assay (ELISA) technique

FDA Status

For research use only, not for use in diagnostic procedures

MSDS

MSDS

notes

The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.

Protocol

Protocol

Sample types

Serum

Sample volume

100 µL of properly diluted unknown / determination

standards

8 standards, serially diluted from 1 prepared lyophilized standard

Standard range

0 / 0.5 - 32 ng/mL

storage

2 - 8 °C

sensitivity

50 pg/mL

Species

Mouse

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Nampt (Visfatin/PBEF) (Intracellular)

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