Human Secretoneurin ELISA
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Secretoneurin, (SN) is a small 33-amino acid neuropeptide detectable in the blood stream and produced by neuroendocrine and heart muscle cells. SN is the only biomarker shown to be associated with biological processes linked to cardiomyocyte Ca2+ handling.<br><br>
The Human Secretoneurin ELISA is a sandwich Enzyme-linked Immunoassay (ELISA). Two secretoneurin-specific sheep monoclonal antibodies (MABs) are used in the assay. One MAB is biotinylated and the second is HRP-conjugated. The biotinylated antibody is added to the wells of a streptavidin coated microtiter plate. After washing, diluted calibrators, controls and samples are added, and secretoneurin present is bound to the immobilized antibody. After incubation of samples the wells are washed to remove unbound sample material, and the HRP-conjugated antibody is added. After incubation and removal of unbound conjugate by washing, the TMB substrate is added. The blue color developed is directly proportional to the amount of secretoneurin present in the calibrators, controls, and samples. The stop solution changes the color from blue to yellow, and the intensity of the color is measured in a microtiter plate reader.
Secretoneurin, (SN) is a small 33-amino acid neuropeptide detectable in the blood stream and produced by neuroendocrine and heart muscle cells. SN is the only biomarker shown to be associated with biological processes linked to cardiomyocyte Ca2+ handling.<br><br>
The Human Secretoneurin ELISA is a sandwich Enzyme-linked Immunoassay (ELISA). Two secretoneurin-specific sheep monoclonal antibodies (MABs) are used in the assay. One MAB is biotinylated and the second is HRP-conjugated. The biotinylated antibody is added to the wells of a streptavidin coated microtiter plate. After washing, diluted calibrators, controls and samples are added, and secretoneurin present is bound to the immobilized antibody. After incubation of samples the wells are washed to remove unbound sample material, and the HRP-conjugated antibody is added. After incubation and removal of unbound conjugate by washing, the TMB substrate is added. The blue color developed is directly proportional to the amount of secretoneurin present in the calibrators, controls, and samples. The stop solution changes the color from blue to yellow, and the intensity of the color is measured in a microtiter plate reader.