Sandwich enzyme-linked immune-sorbent assay (ELISA) for the determination of GH in human serum, plasma, body fluids, tissue homogenate or cell culture supernates. For research use only, not for use in diagnostic procedures.<br><br>
Growth hormone (GH) is a peptide hormone that stimulates growth, cell reproduction and regeneration in humans and other animals. It is synthesized by acidophilic or somatotropic cells of the anterior pituitary gland. Human growth hormone has a molecular mass of 22,005 and contains 191 amino acid residues with 2 disulfide bridges. Growth hormone is used as a prescription drug in medicine to treat children's growth disorders and adult growth hormone deficiency. In its role as an anabolic agent, HGH has been abused by competitors in sports since the 1970s, and it has been banned by the IOC and NCAA.
- Assay Description:
- 90 min. incubation (37°C) + 60 min. (37°C) + 30 min. (37°C) + 30 min. (37°C) = 3 hours, 30 min. total incubation time
- Catalog number:
- BE69136
- configuration:
- 96 Determinations, 12x8 removable strips
- controls:
- None provided
- design:
- Sandwich enzyme-linked immunesorbent assay (ELISA) technique
- FDA Status:
- For research use only, not for use in diagnostic procedures
- notes:
- The protocol for this product (see above) is intended to serve as an example only. Please refer to the Instructions For Use provided with the assay kit for precise details.
- Protocol:
- Sample types:
- Human serum, plasma, body fluids, tissue homogenate or cell culture supernates
- Sample volume:
- 100 μL of properly diluted unknown / determination
- standards:
- 8 standards, serially diluted from 1 prepared lyophilized standard
- Standard range:
- 0 / 15.6 - 1000.0 pg/mL
- storage:
- 2 - 8 °C
- sensitivity:
- <2 pg/mL
- Species:
- Human
