behars Loading... Please wait...

Anti-Smad3L (S213 Phosphorylated) (5A11) Mouse IgG MoAb

Product Description

Phosphorylation of signal transduction molecules Smad3, can be an important information for understanding of various biological functions of transforming growth factor (TGF)-β. TGF-β type I receptor and mitogen activated protein kinase (MAPK) phosphorylate Smad3 at the C-terminals and at linker (middle) regions respectively (ref. 1, 2). Mitogenic signaling induced by HGF, EGF and inflammatory cytokines is mediated by Smad3 phosphoisoform phophorylated serine located in linker region (ref. 3). This monoclonal antibody against for Smad3L (S213) recognizes the part of phosphorylated serine (Ser213) located in linker region of Smad3 specifically. It can be used for western blotting, immunoprecipitation and immunohistochemistry. It is applicable for enzyme biochemical analysis of Smad3 signaling and makes it possible to visualize the intermolecular reaction of phosphorylated Smad3 signaling in human tissues and monitor them in real-time. Thus, analysis of Smad3 signaling with this antibody is expected to be widely applied to cancer serearch (ref. 3) and fibrosis research (ref. 4), and to contribute to understanding the wide variety of life phenomina mediated by phosphoisoforms of Smad3.

List Price: $499.00
QTY
SKU:
10387

Product Description

Phosphorylation of signal transduction molecules Smad3, can be an important information for understanding of various biological functions of transforming growth factor (TGF)-β. TGF-β type I receptor and mitogen activated protein kinase (MAPK) phosphorylate Smad3 at the C-terminals and at linker (middle) regions respectively (ref. 1, 2). Mitogenic signaling induced by HGF, EGF and inflammatory cytokines is mediated by Smad3 phosphoisoform phophorylated serine located in linker region (ref. 3). This monoclonal antibody against for Smad3L (S213) recognizes the part of phosphorylated serine (Ser213) located in linker region of Smad3 specifically. It can be used for western blotting, immunoprecipitation and immunohistochemistry. It is applicable for enzyme biochemical analysis of Smad3 signaling and makes it possible to visualize the intermolecular reaction of phosphorylated Smad3 signaling in human tissues and monitor them in real-time. Thus, analysis of Smad3 signaling with this antibody is expected to be widely applied to cancer serearch (ref. 3) and fibrosis research (ref. 4), and to contribute to understanding the wide variety of life phenomina mediated by phosphoisoforms of Smad3.

Other Details

application:
IHC, WB, Immuno-precipitation.
Catalog number:
10387
clone:
5A11
concentration:
Please see datasheet.
Datasheet:
formulation:
Lyophilized product in PBS containing 1 % BSA and 0.05 % NaN3
immunogen:
Synthetic peptide of phosphorylated Smad3L (S213).
isotype:
IgG1
notes:
For research use only, not for use in diagnostic procedures.
The datasheet for this product (see above) is intended to serve as an example only. Please refer to the datasheet provided with the antibody for precise details.
Other names:
Please see datasheet.
size:
50 μg
storage:
Lyophilized product, 5 years at 2 - 8 °C; Solution, 2 years at –20 °C.
Host:
Mouse

OUR ASSAY KIT CATEGORIES

Back to Top